Multiclamp 700b commander

Ion channels are pore proteins that are involved in a large variety of physiological processes. Basic research in the ion channel field furthers the understanding of ion channel function as well as their roles within cellular pathways and processes. Single-channel research helps with elucidating the mechanisms underlying ion channel function on a single-molecule level.

Whole-cell and perforated patch clamp recordings are typically used to investigate the action of modulators on entire populations of ion channels in the membrane of a cell.

AxonInstruments MultiClamp 700B

Ion channels are involved in many cell pathways and understanding the function of ion channels in response to changes in membrane potential or the presence or absence of other molecules is important in order to understand exactly how ion channels participate in normal and abnormal biological processes such as cell differentiation and migration, disease states, neuronal communications, etc.

Ion channels play a role in many diseases, including hypertension, cardiac arrhythmias, gastrointestinal, immune and neuromuscular disorders, pathological pain and cancer.

By understanding the exact role that ion channels play in a particular disease, researchers might be able to find a way to affect the ion channel in such a way as to alter the course of the disease.

A critical part of drug discovery involves safety assessments, such as hERG testing, in order to eliminate any compounds with potential safety concerns.

Conventional patch clamp has played an important role in collecting evidence for the absence of side effects before submission of a drug candidate to regulatory authorities. Even if automated electrophysiology has shifted safety testing to earlier stages in the drug discovery process, follow-up with conventional patch clamp is an essential step in the workflow. Instead of the traditional front panel knobs and switches, the Axoclamp A Amplifier is controlled by the Axoclamp A Commander Software, a program that runs on a Windows PC computer see system requirements and communicates with the amplifier via a USB interface.

Axoclamp 900A

This control interface reports resistance, voltage, and current measurements and provides automation of Bridge Balance, Pipette Offset, and Pipette Capacitance Neutralization for improved ease of use. This ensures that the amplifier settings are accurately and conveniently stored in the recorded data files.

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Computer control of the amplifier ensures correct scaling and logging of experimental parameters. Email address:. Axoclamp A Microelectrode Amplifier.

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Add to quote list. Category: Oocyte Amplifiers. Overview Applications Software Specifications Order Overview For two-electrode voltage clamp recordings, whole-cell current-clamp recordings, intracellular sharp electrode recordings, extracellular field potential recordings in ion channel, neuroscience, oocyte and iontophoresis studies. This amplifier features many enhancements that improve its recording capability, make the amplifier easier to use, and your experiments last longer.

The Axoclamp A Microelectrode Amplifier provides the following modes of operation:. Applications Basic Ion Channel Research. Cell Pathway Analysis. Disease Research. Safety Assessment. Software Instead of the traditional front panel knobs and switches, the Axoclamp A Amplifier is controlled by the Axoclamp A Commander Software, a program that runs on a Windows PC computer see system requirements and communicates with the amplifier via a USB interface.

Specifications General Specifications Dimensions in.Page of Go. Quick Links. Download this manual. MultiClamp B. No part of this manual may be reproduced, stored in a retrieval system, or. Table of Contents. No part of this manual may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means, electronic, mechanical, photocopying, microfilming, recording, or otherwise, without written permission from Molecular Devices Corp. Page 3!!!!!

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Please carefully read the safety warnings starting on page before you use this instrument. Page 6 Bridge Balance Page 8 Changing the Fuse Thus, BNC-type input and output connections are necessary to communicate with a digitizing interface, oscilloscope or other recording device.

Page 12 We will be pleased to answer any questions regarding the theory and use of the MultiClamp B. Any comments and suggestions on the use and design of the MultiClamp B will be much appreciated. We welcome reprints of papers describing work performed with the MultiClamp B.

Page Chapter 2 Installation And Basic Operation Retain packing materials in case the instrument needs to be returned to the factory at a later date.

For the initial checkout, the MultiClamp B should be situated on a bench top away from other equipment. Do not install it in a rack until the checkout is complete.

Page Installing Hardware 1. Page 15 Figure 2. If you are using the optional SoftPanel device, click on the SoftPanel tab and click the Scan button. To demonstrate this, press the V-Clamp 1 tab and try the following. Page 18 For example, right-click the mouse while over the Holding glider, and you will see the following menu. Figure 2. Page Calibration 6. If your MultiClamp has more than one CV-7 headstage, repeat steps for the second headstage.

Calibration The steps below provide a quick check of the calibration of the MultiClamp B.A microelectrode amplifier that combines leading edge electronics design and a wealth of features and functions with an intuitive user interface that simplifies the creation of complex protocols. The applications for this amplifier comprise a wide variety of intracellular or extracellular recordings in electrophysiology, including:.

With two independent primary headstages and an additional two auxiliary headstages, the Multiclamp B Microelectrode Amplifier houses the solution for almost any conceivable electrophysiology recording in one slim, convenient package. Instead of front panel knobs and switches, the MultiClamp B Amplifier is controlled by Multiclamp B Commander software, a program that runs on a host computer PC and Macintosh; see Requirements below and communicates with the amplifier via a USB interface.

The intuitive user interface of MultiClamp Commander gives the operator full control of all amplifier functions, while the Quick Select feature enables easy access to saved configurations that apply to particular recording configurations.

The optional, user-configurable SoftPanel lets you operate the most commonly used controls using traditional knobs and buttons. The Multiclamp B Amplifier comes standard with two independent CV-7B headstages, each of which contains both current-to-voltage and voltage-following circuitry. This design allows the user to rapidly switch between patch clamp recording and true high-speed current clamp recording. Thus, with two headstages, the Multiclamp B Amplifier can perform the function of two patch clamp amplifiers, two current clamp amplifiers, or a combination of patch and current clamp amplifiers.

Furthermore, two optional voltage-follower headstages HS-2 type can be connected to auxiliary inputs to allow third- and fourth-point voltage recording. Major new features of the Multiclamp B Amplifier include Internal Automatic Mode switching, triggered by external signals or the recording itself; Oscillation Suppression for better cell protection; and Slow Current Injection to compensate for drifting potentials.

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For more information and a full listing of features and specifications, go to the Specifications tab below or download the manual in the Downloads section. Find new and used pricing to meet your lab needs. Choose from top brands in our marketplace.

Molecular Devices Axon Multiclamp B A microelectrode amplifier that combines leading edge electronics design and a wealth of features and functions with an intuitive user interface that simplifies the creation of complex protocols. Refine By Listing Type Request a Quote 1. Clear Filters. Showing 1 - 1 of 1 Items. Show 24 32 64 Instead of the usual front panel knobs and switches, the Axoclamp A is controlled by the Axoclamp A Commander, a program that runs on a Windows PC computer see Requirements below and communicates with the amplifier via a USB 2.

This control interface reports resistance, voltage, and current measurements and provides automation of Bridge Balance, Pipette Offset, and Pipette Capacitance Neutralization. The optional SoftPanel was designed for those who prefer the more conventional feel to amplifier control of knobs and buttons. By way of a USB connection, the SoftPanel physically replicates all essential amplifier functions by acting as a hardware extension of the Axoclamp A Commander software.

MultiClamp BThe Axoclamp A is a complete microelectrode current-clamp and voltage-clamp amplifier, useful for a wide range of intracellular microelectrode recording techniques. The Axoclamp A has several modes of operation:.

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Note: This amplifier does not have continuous single-electrode voltage clamp mode. However, this mode is available in our patch-clamp amplifiers: the MultiClamp B and Axopatch B.

Please see the Amplifier comparison chart for more details on the features of our amplifiers. Two USB 2.

Molecular Devices Axon Multiclamp 700B

Thus, it requires a separate system for controlling stimulus protocols and recording the output, such as a digitizer and data acquisition software. The Digidata digitizer and pCLAMP 10 data acquisition and analysis software from Molecular Devices, work together to provide the most complete, integrated solution. Excellent Comprehensive Manual We also provide a detailed User Guide that serves as a handbook of procedures for microelectrode users. Tutorials written by Axon Instruments staff and scientific consultants provide a useful guide to the operation of the instrument and are informative references for several electrophysiological techniques.

Molecular Devices Axoclamp A Amplifier. Computer-Controlled High-Compliance Current Clamp and Voltage Clamp MultiClamp BThe Axoclamp A is a complete microelectrode current-clamp and voltage-clamp amplifier, useful for a wide range of intracellular microelectrode recording techniques. The Axoclamp A has several modes of operation: I-Clamp: two independent bridge amplifiers for voltage measurements DCC: discontinuous current clamp for accurate voltage measurements, even when electrode resistance changes TEVC: high-compliance two-electrode voltage clamp for oocytes and mammalian cells dSEVC: discontinuous single-electrode voltage clamp for small cells with large currents HVIC: high-voltage current clamp for extracellular applications such as iontophoresis Note: This amplifier does not have continuous single-electrode voltage clamp mode.

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Buy Now. Molecular Devices Video Webinar.Multiclamp Commander must be running before starting ACQ4. A MultiClamp device in ACQ4 represents only a single channel; to access both channels, simply configure two devices with the appropriate channelID strings. The holding levels configured by the Commander software are separate and will be added to any holding level or output commands specified in ACQ4.

Optionally, a second DAQ analog input may be used to record the secondary output per MultiClamp channel. The following example is for channel 1 on a Multiclamp A.

To use two channels, simply define a second device with the appropriate channelID string. For A devices the string is formatted like model:MCA,com:3,dev:0,chan For B devices, the string is formatted like model:MCB,sn,chan To determine the channelIDs available on your system, simply start ACQ4 with a MultiClamp device configured with an empty channelID string; an error message will be displayed containing the available strings.

This may be used to ensure that experiments always begin with a consistent configuration especially gain and filter settings. Parameters are split up into IC and VC groups; see above for an example.

For a complete listing of the parameters available, open a Console module and type the following:. This will print the complete set of parameters currently active on the device.

multiclamp 700b commander

This interface allows the design of stimulation waveforms and recording parameters for executing tasks. Command function holds a standard function generator that is used to specify the output waveform and sequence parameters.

The right half of the interface holds plot areas for displaying the recorded primary signal top and command signal bottom. Metadata are as follows:. Patch-Clamp Amplifiers. AxoPatch Devices. Navigation index next previous ACQ4 0. Recording all MultiClamp state parameters along with physiological data. The amplifier will be reconfigured with these options every time ACQ4 is started.

Metadata are as follows: All DAQ configuration settings including sample rate, filtering, and downsampling. The standard function generator metadata structure. Quick search. Created using Sphinx 1.Many automated functions and effective signal conditioning. Add two more optional headstage voltage-follower inputs for a four-point recording system, ideal for complex synaptic recordings.

Major new features of the MultiClamp B Amplifier include Internal Automatic Mode switching, triggered by external signals or the recording itself; Oscillation Suppression for better cell protection; and Slow Current Injection to compensate for drifting potentials. Ion channels are pore proteins that are involved in a large variety of physiological processes.

Basic research in the ion channel field furthers the understanding of ion channel function as well as their roles within cellular pathways and processes. Single-channel research helps with elucidating the mechanisms underlying ion channel function on a single-molecule level. Whole-cell and perforated patch clamp recordings are typically used to investigate the action of modulators on entire populations of ion channels in the membrane of a cell. Ion channels are involved in many cell pathways and understanding the function of ion channels in response to changes in membrane potential or the presence or absence of other molecules is important in order to understand exactly how ion channels participate in normal and abnormal biological processes such as cell differentiation and migration, disease states, neuronal communications, etc.

Ion channels play a role in many diseases, including hypertension, cardiac arrhythmias, gastrointestinal, immune and neuromuscular disorders, pathological pain and cancer. By understanding the exact role that ion channels play in a particular disease, researchers might be able to find a way to affect the ion channel in such a way as to alter the course of the disease. Learn more. A critical part of drug discovery involves safety assessments, such as hERG testing, in order to eliminate any compounds with potential safety concerns.

Conventional patch clamp has played an important role in collecting evidence for the absence of side effects before submission of a drug candidate to regulatory authorities. Even if automated electrophysiology has shifted safety testing to earlier stages in the drug discovery process, follow-up with conventional patch clamp is an essential step in the workflow. This control interface reports resistance, voltage, and current measurements and provides automation of Bridge Balance, Pipette Offset, and Pipette Capacitance Neutralization for improved ease of use.

Quick Select provides easy access to stored preset configurations that apply to particular applications. This ensures that the amplifier settings are accurately and conveniently stored in the recorded data files. Computer control of the amplifier ensures correct scaling and logging of experimental parameters.

multiclamp 700b commander

To order the Multiclamp B amplifier please Call us at: or email us at: sales alascience. Email address:. MultiClamp B Patch voltage-clamp amplifier. Add to quote list. Category: Patch Clamp Amplifiers. Applications Ion channels are pore proteins that are involved in a large variety of physiological processes.

Cell Pathway Analysis Ion channels are involved in many cell pathways and understanding the function of ion channels in response to changes in membrane potential or the presence or absence of other molecules is important in order to understand exactly how ion channels participate in normal and abnormal biological processes such as cell differentiation and migration, disease states, neuronal communications, etc.

Molecular Devices Axoclamp 900A Amplifier

Disease Research Ion channels play a role in many diseases, including hypertension, cardiac arrhythmias, gastrointestinal, immune and neuromuscular disorders, pathological pain and cancer. Learn more Safety Assessment A critical part of drug discovery involves safety assessments, such as hERG testing, in order to eliminate any compounds with potential safety concerns. Specifications General Specifications Dimensions in. Order To order the Multiclamp B amplifier please Call us at: or email us at: sales alascience.August 31, at pm - Permalink.

September 1, at am - Permalink. August 22, at pm - Permalink. October 25, at am - Permalink. AxonInstruments MultiClamp B. Since Igor 6. However the XOP does not allow you to directly control the device. To do that, you still need to use the MultiClamp Commander program that Axon ships with the amplifier.

multiclamp 700b commander

Execute the following command in Igor to bring up the help file for the XOP. Even though the XOP can only read "telegraphs" from the amplifier, you still control other aspects of the amplifier with Igor via your digitizer.

This can be used to control the amplifier commands, as well as the amplifier mode voltage clamp vs. In my experience, there was something flaky about controlling the mode in this way as opposed to through Multiclamp Commander Thank you both! That helps a lot. I am new to programming and it is not often intuitive to me how to approach solutions to tasks such as this one.

I really appreciate the support. Hello guys I am not able to set the Amplifier settings for the Multiclamp B commander in order to get the exact holding voltage that i am entering. Also If i set the holding voltage to say a set value of mV then for that setting of the amplifier the potential that we want to set gets multiplied by a factor of 10 or so.

So please can any of you tell me any solution to this problem.


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